The Acetylene-Ethylene Assay for N2 Fixation: Laboratory and Field Evaluation
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منابع مشابه
The acetylene-ethylene assay for n(2) fixation: laboratory and field evaluation.
The methodology, characteristics and application of the sensitive C(2)H(2)-C(2)H(4) assay for N(2) fixation by nitrogenase preparations and bacterial cultures in the laboratory and by legumes and free-living bacteria in situ is presented in this comprehensive report. This assay is based on the N(2)ase-catalyzed reduction of C(2)H(2) to C(2)H(4), gas chromatographic isolation of C(2)H(2) and C(2...
متن کاملSimultaneous measurement of nitrogen fixation estimated by acetylene-ethylene assay and nitrate absorption by soybeans.
An apparatus was designed for simultaneous measurement of rates of N(2) fixation estimated by C(2)H(2)-C(2)H(4) assay (N(2)[C(2)H(2)] fixation) and NO(3) (-) absorption by roots of intact, nodulated soybeans (Glycine max [L.] Merr.). The principal design features include: (a) a gas-tight mist chamber in which nodulated roots can be exposed simultaneously to C(2)H(2) in the gas phase and to a li...
متن کاملIn situ studies on N2 fixation using the acetylene reduction technique.
The measurement of in situ N2-fixation rates on the basis of total nitrogen changes or 15N2 uptake is not entirely satisfactory-the former method is insufficiently sensitive and accurate, and the '5N method is time consuming, expensive, and requires a mass spectrometer. The discovery in 1966 by Sch6llhorn and Burris' and by Dilworth2 that the nitrogen-fixing complex (nitrogenase) reduces acetyl...
متن کاملThe acetylene reduction assay for measuring nitrogen fixation in waterlogged soil.
Nitrogen fixation in waterlogged, soil-straw, and sandday-straw mixtures was measured with the C2H2reduction assay, the I5N-tracer technique, and the Kjeldahl method. The reduction of 6 to 15 moles C2H2 corresponded to the fixation of 1 mole N2. The theoretical ratio is 3 moles C2H2 to 1 mole N2. A ratio of 3 moles C2H2 reduced for each mole of N2 fixed was obtained when samples of sandxlay-str...
متن کاملFate of N2 fixation
Introduction Conclusions References Tables Figures Back Close Full Screen / Esc Abstract Introduction Conclusions References Tables Figures Back Close Full Screen / Esc Printer-friendly Version Interactive Discussion EGU Abstract While we now know that marine N 2 fixation is a significant source of new nitrogen (N) in the marine environment, little is known about the fate of this production, de...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1968
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.43.8.1185